Connexin 43/GJA1 antibody

Catalog No. ABIN641009

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody has been validated for WB, ELISA and Func. It is suitable to detect Connexin 43/GJA1 in samples from Chicken.

Quick Overview for Connexin 43/GJA1 antibody (ABIN641009)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Product Details anti-Connexin 43/GJA1 Antibody

Purpose: Rabbit Anti-Chicken Connexin 43 (pSer368)

Specificity: This product recognizes chicken Interferon gamma (chIFN-gamma). Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFN-gamma strongly upregulates the expression of MHC class II and iNOS genes. It can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (2).

Characteristics: Adhesion Molecule/Cell Surface

Immunogen: Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.

Isotype: IgG

Application Details

Application Notes: ELISA, Functional Assays, Western Blotting

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 0.5 mg/ml

Buffer: Phosphate buffered saline

Preservative: Azide free

Storage: -20 °C

Storage Comment: -20°C

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization